Hotspot

petD-rpoA

LSC · 82371-82488 · 118 bp

Back to Haloragis

Pi 0.0424

MarkerSeek score 76.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 5
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGTTGGGTATTGGAGCAAC	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	253-307 bp	1.0	58.6
petD-rpoA_p2	TGGGTATTGGAGCAACCTTG	TTGGGCATTCTAGAAAAGCA	50.0	40.0	57.8	55.2	249-303 bp	1.0	57.5
petD-rpoA_p3	GTTGGGTATTGGAGCAACCT	TTGGGCATTCTAGAAAAGCA	50.0	40.0	57.8	55.2	251-305 bp	1.0	57.5
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	TGGGCATTCTAGAAAAGCAT	50.0	40.0	58.1	55.0	252-306 bp	1.0	56.7
petD-rpoA_p5	TGGGTATTGGAGCAACCTTG	TGGGCATTCTAGAAAAGCAT	50.0	40.0	57.8	55.0	248-302 bp	1.0	55.6