Hotspot

atpB-rbcL

LSC · 58470-59296 · 827 bp

Back to Haloragis

Pi 0.00607

MarkerSeek score 62.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 5
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATTTGAGTGATCCGTCCCG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	954-959 bp	1.0	72.3
atpB-rbcL_p2	AATTTGAGTGATCCGTCCCG	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	953-958 bp	1.0	72.1
atpB-rbcL_p3	GCCTGGGGAAAAAGCTACAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	990-995 bp	1.0	71.3
atpB-rbcL_p4	GCTACATCCAATACCGGACC	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	977-982 bp	1.0	71.2
atpB-rbcL_p5	GCCTGGGGAAAAAGCTACAT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	989-994 bp	1.0	71.1