Hotspot

ndhC-trnV-UAC

LSC · 54479-55436 · 958 bp

Back to Haloragis

Pi 0.0123

MarkerSeek score 70.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 11
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCGGAAATGAGAAATGCCA	GGTTCGAATCCGTATAGCCC	45.0	55.0	57.5	57.9	1011-1067 bp	1.0	80.2
ndhC-trnV-UAC_p2	TCCGGAAATGAGAAATGCCA	CGGTTCGAATCCGTATAGCC	45.0	55.0	57.5	58.3	1012-1068 bp	1.0	78.4
ndhC-trnV-UAC_p3	TCCTTTGCGAATTGGAGCTA	GGTTCGAATCCGTATAGCCC	45.0	55.0	57.2	57.9	1035-1091 bp	1.0	78.0
ndhC-trnV-UAC_p4	TCCGGAAATGAGAAATGCCA	ACTGCATGTTGGGTCTTTGA	45.0	45.0	57.5	57.6	1089-1145 bp	1.0	77.8
ndhC-trnV-UAC_p5	GTCCTTTGCGAATTGGAGCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.8	57.9	1036-1092 bp	1.0	77.6