Hotspot

trnC-GCA-trnT-GGU

LSC · 32942-34046 · 1105 bp

Back to Haloragis

Pi 0.0275

MarkerSeek score 77.00

Primer available yes

Label trnC-GCA-trnT-GGU

Feature Metadata

label_name: trnC-GCA-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 29
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnT-GGU_p1	CCGGATTTGAACTGGGGAAA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1222-1369 bp	1.0	84.3
trnC-GCA-trnT-GGU_p2	CCGGATTTGAACTGGGGAAA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	58.1	57.9	1187-1334 bp	1.0	83.7
trnC-GCA-trnT-GGU_p3	CCGGATTTGAACTGGGGAAA	CCGATGACTTACGCCTTACC	50.0	55.0	58.1	58.2	1219-1366 bp	1.0	83.1
trnC-GCA-trnT-GGU_p4	AAAAGGATTTGCAGTCCCCC	GAACCGATGACTTACGCCTT	50.0	50.0	58.4	58.0	1204-1351 bp	1.0	82.2
trnC-GCA-trnT-GGU_p5	CCGGATTTGAACTGGGGAAA	TTGAACCGATGACTTACGCC	50.0	50.0	58.1	58.3	1224-1371 bp	1.0	82.1