Hotspot

psbM-petN

LSC · 31391-32313 · 923 bp

Back to Haloragis

Pi 0.022
MarkerSeek score 72.70
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
923
variable_sites
20
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTTATTCT TAGTAAGTCTCGCTTGGGCT 38.1 50.0 55.2 58.2 1091-1093 bp 1.0 51.1
psbM-petN_p2 TGCTACTGCACTGTTTATTCT ATGGGGAAGAAGTGGACTCT 38.1 50.0 55.2 57.6 1030-1032 bp 1.0 49.7
psbM-petN_p3 TGCTACTGCACTGTTTATTC TAGTAAGTCTCGCTTGGGCT 40.0 50.0 53.6 58.2 1091-1093 bp 1.0 46.3
psbM-petN_p4 TGCTACTGCACTGTTTATTCT ATAGTAAGTCTCGCTTGGGC 38.1 50.0 55.2 57.1 1092-1094 bp 1.0 45.2
psbM-petN_p5 TGCTACTGCACTGTTTATTCT CTCGCTTGGGCTGCTTTAAT 38.1 50.0 55.2 58.9 1083-1085 bp 1.0 45.2