Hotspot

rpoB-trnE-UUC

LSC · 29037-29678 · 642 bp

Back to Haloragis

Pi 0.00656

MarkerSeek score 67.30

Primer available yes

Label rpoB-trnE-UUC

Feature Metadata

label_name: rpoB-trnE-UUC
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 4
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnE-UUC_p1	CGTCAAGCCCTGATCAAGAA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.9	58.3	729-761 bp	1.0	81.0
rpoB-trnE-UUC_p2	CGTCAAGCCCTGATCAAGAA	TTTCTTTCGCCGGATCTCAC	50.0	50.0	57.9	58.3	771-803 bp	1.0	80.6
rpoB-trnE-UUC_p3	AAAGTTCTTCCGTCAAGCCC	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.4	58.3	739-771 bp	1.0	78.6
rpoB-trnE-UUC_p4	CGTCAAGCCCTGATCAAGAA	CGGGAAGGCGAATTTCTTTC	50.0	50.0	57.9	57.5	783-815 bp	1.0	78.5
rpoB-trnE-UUC_p5	AAAGTTCTTCCGTCAAGCCC	TTTCTTTCGCCGGATCTCAC	50.0	50.0	58.4	58.3	781-813 bp	1.0	78.2