Hotspot

atpH-atpI

LSC · 15598-16764 · 1167 bp

Back to Haloragis

Pi 0.00431

MarkerSeek score 61.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAAGTCCTACGGCTAATCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1275-1282 bp	1.0	75.5
atpH-atpI_p2	CAGGGCCAATAGAAGCAAGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1289-1296 bp	1.0	75.5
atpH-atpI_p3	AGAAGCAAGTCCTACGGCTA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.2	58.5	1279-1286 bp	1.0	75.4
atpH-atpI_p4	TAGAAGCAAGTCCTACGGCT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.2	58.5	1280-1287 bp	1.0	75.4
atpH-atpI_p5	ATCCAGCAGCAATAACGGAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	1259-1266 bp	1.0	74.0