Hotspot

ycf2

IRa · 148862-155488 · 6627 bp

Back to Halophila

Pi 0.0955

MarkerSeek score 75.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6627
variable_sites: 604
pi: 0.0955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATTCCGCAAGACCAAGATCC	AGAAGTGACGCGGATGATTC	50.0	50.0	58.0	58.1	1878-2016 bp	1.0	91.1
ycf2_p2	ATTCCGCAAGACCAAGATCC	TTTGGGTTGGAGCAGATTCC	50.0	50.0	58.0	58.1	2486-2627 bp	1.0	91.0
ycf2_p3	ATTCCGCAAGACCAAGATCC	AAGTGACGCGGATGATTCAA	50.0	45.0	58.0	57.9	1876-2014 bp	1.0	90.9
ycf2_p4	ATTCCGCAAGACCAAGATCC	AACCCATGTTTGGACGGAAA	50.0	45.0	58.0	57.9	2466 bp	0.5	59.0
ycf2_p5	ATTCCGCAAGACCAAGATCC	GTGGGAACATTCGGGTCTTT	50.0	50.0	58.0	58.1	1708 bp	0.5	58.9