Hotspot

ycf1

IRb · 115463-122206 · 6744 bp

Back to Halophila

Pi 0.149

MarkerSeek score 75.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 6744
variable_sites: 863
pi: 0.149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAAAATTGCGTTTGCCACA	GTCTTCGTCTTCTGCGTCTT	40.0	50.0	58.0	57.9	2519 bp	0.5	59.4
ycf1_p2	ACAAAATTGCGTTTGCCACA	TTCTCCTCCTCACTCATGCA	40.0	50.0	58.0	58.1	2065 bp	0.5	59.4
ycf1_p3	AATCCGTGGATTGTCTTCGG	GTCTTCGTCTTCTGCGTCTT	50.0	50.0	58.0	57.9	1537 bp	0.5	59.4
ycf1_p4	CCGTGGATTGTCTTCGGAAT	GTCTTCGTCTTCTGCGTCTT	50.0	50.0	58.0	57.9	1534 bp	0.5	59.4
ycf1_p5	ACAAAATTGCGTTTGCCACA	CCTTTGATTAGACCCCGACG	40.0	55.0	58.0	58.1	2593 bp	0.5	59.4