Hotspot

accD

LSC · 58789-62217 · 3429 bp

Back to Halophila

Pi 0.164

MarkerSeek score 70.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 3429
variable_sites: 350
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GACGGATCAGCTTGCCTTAA	GGACCAAACGATCCGAATCA	50.0	50.0	58.0	58.0	530 bp	0.5	59.7
accD_p2	GACGGATCAGCTTGCCTTAA	ACCAAACGATCCGAATCAGG	50.0	50.0	58.0	58.0	528 bp	0.5	59.6
accD_p3	AAAGGGGGCTTGAAAGATGG	GGACCAAACGATCCGAATCA	50.0	50.0	58.1	58.0	783 bp	0.5	59.4
accD_p4	AAAGGGGGCTTGAAAGATGG	ACCAAACGATCCGAATCAGG	50.0	50.0	58.1	58.0	781 bp	0.5	59.3
accD_p5	GGGCTTGAAAGATGGTTGGA	GGACCAAACGATCCGAATCA	50.0	50.0	58.1	58.0	778 bp	0.5	59.2