Hotspot

rpoA-trnH-GUG

IRa · 168400-168457 · 58 bp

Back to Halophila

Pi 0.0877

MarkerSeek score 76.60

Primer available yes

Label rpoA-trnH-GUG

Feature Metadata

label_name: rpoA-trnH-GUG
feature_type: igs
region: IRa
length_bp: 58
variable_sites: 5
pi: 0.0877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-trnH-GUG_p1	CGTTTCGCAATTGATTTACCT	TGGATTAAGGCGGTGGATTG	38.1	50.0	55.5	57.9	158-159 bp	1.0	54.7
rpoA-trnH-GUG_p2	CGTTTCGCAATTGATTTACCT	GGATTAAGGCGGTGGATTGT	38.1	50.0	55.5	57.9	157-158 bp	1.0	54.7
rpoA-trnH-GUG_p3	CGTTTCGCAATTGATTTACCT	GTGGATTAAGGCGGTGGATT	38.1	50.0	55.5	57.9	159-160 bp	1.0	54.7
rpoA-trnH-GUG_p4	CGTTTCGCAATTGATTTACCT	GATACATTCCAAGGGACGGG	38.1	55.0	55.5	57.7	192-193 bp	1.0	52.6
rpoA-trnH-GUG_p5	CGTTTCGCAATTGATTTACCT	CGGATGTAGCCAAGTGGATT	38.1	50.0	55.5	57.7	172-173 bp	1.0	52.2