Hotspot

rps7-ycf2

IRa · 148136-148861 · 726 bp

Back to Halophila

Pi 0.17

MarkerSeek score 80.40

Primer available yes

Label rps7-ycf2

Feature Metadata

label_name: rps7-ycf2
feature_type: igs
region: IRa
length_bp: 726
variable_sites: 102
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ycf2_p1	CGAGCCGTGAAAGAGATTCA	TCAGCAACCCAAAGTCGAAA	50.0	45.0	58.0	58.0	1205 bp	0.5	59.6
rps7-ycf2_p2	CGAGCCGTGAAAGAGATTCA	TTCAGCAACCCAAAGTCGAA	50.0	45.0	58.0	58.0	1206 bp	0.5	59.6
rps7-ycf2_p3	CTGTGGAAAGCCGTATTCGA	TCAGCAACCCAAAGTCGAAA	50.0	45.0	58.0	58.0	1500 bp	0.5	59.6
rps7-ycf2_p4	CTGTGGAAAGCCGTATTCGA	TTCAGCAACCCAAAGTCGAA	50.0	45.0	58.0	58.0	1501 bp	0.5	59.6
rps7-ycf2_p5	TCTGTGGAAAGCCGTATTCG	TCAGCAACCCAAAGTCGAAA	50.0	45.0	58.0	58.0	1501 bp	0.5	59.6