Hotspot

ycf1-rpl32

IRa · 134005-134878 · 874 bp

Back to Halophila

Pi 0.276

MarkerSeek score 81.10

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: IRa
length_bp: 874
variable_sites: 205
pi: 0.276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	TGTGGCAAACGCAATTTTGT	ATGGGTTTACTCCACCAGGA	40.0	50.0	58.0	57.9	1938-2914 bp	1.0	91.4
ycf1-rpl32_p2	ATTCCCTAGGACCCAACCAA	ATGGGTTTACTCCACCAGGA	50.0	50.0	57.9	57.9	1968-2944 bp	1.0	91.0
ycf1-rpl32_p3	TGTGGCAAACGCAATTTTGT	AACATTTGTTGCCTGATGCG	40.0	45.0	58.0	57.9	1659 bp	0.5	59.3
ycf1-rpl32_p4	ACCGCGAATTTATGAGGTCC	ATGGGTTTACTCCACCAGGA	50.0	50.0	58.1	57.9	1646 bp	0.5	59.2
ycf1-rpl32_p5	ACCGCGAATTTATGAGGTCC	AACATTTGTTGCCTGATGCG	50.0	45.0	58.1	57.9	1367 bp	0.5	59.0