Hotspot

trnL-UAG-ycf1

SSC · 126590-127260 · 671 bp

Back to Halophila

Pi 0.293

MarkerSeek score 82.90

Primer available yes

Label trnL-UAG-ycf1

Feature Metadata

label_name: trnL-UAG-ycf1
feature_type: igs
region: SSC
length_bp: 671
variable_sites: 191
pi: 0.293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ycf1_p1	GGACTTGAACCGAGATGCTT	AGCCCAATACCCAAATCGAC	50.0	50.0	57.9	57.9	1378 bp	0.5	58.8
trnL-UAG-ycf1_p2	TCCTAAGAGCAGCGTGTCTA	AGCCCAATACCCAAATCGAC	50.0	50.0	57.9	57.9	1348 bp	0.5	58.6
trnL-UAG-ycf1_p3	GGACTTGAACCGAGATGCTT	CCACTCCGTGCATTTAAGGA	50.0	50.0	57.9	57.9	1295 bp	0.5	58.3
trnL-UAG-ycf1_p4	GGACTTGAACCGAGATGCTT	CGTGCATTTAAGGACTGGGA	50.0	50.0	57.9	57.9	1289 bp	0.5	58.3
trnL-UAG-ycf1_p5	GGACTTGAACCGAGATGCTT	TCCGTGCATTTAAGGACTGG	50.0	50.0	57.9	57.9	1291 bp	0.5	58.3