Hotspot

psaC-ccsA

SSC · 123990-125436 · 1447 bp

Back to Halophila

Pi 0.151

MarkerSeek score 65.30

Primer available yes

Label psaC-ccsA

Feature Metadata

label_name: psaC-ccsA
feature_type: igs
region: SSC
length_bp: 1447
variable_sites: 80
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.37
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ccsA_p1	AGATGTGAATCCGCTTGTCC	CCTATTGGAATTGGGACCCG	50.0	55.0	58.0	58.0	1752 bp	0.5	59.6
psaC-ccsA_p2	AGATGTGAATCCGCTTGTCC	TGGGACCCGAAAGAAACTTG	50.0	50.0	58.0	58.0	1741 bp	0.5	59.6
psaC-ccsA_p3	AGATGTGAATCCGCTTGTCC	TTGGAATTGGGACCCGAAAG	50.0	50.0	58.0	58.1	1748 bp	0.5	59.1
psaC-ccsA_p4	AGATGTGAATCCGCTTGTCC	ACCACAGGCATTCTTTCTGG	50.0	50.0	58.0	58.1	1801 bp	0.5	59.1
psaC-ccsA_p5	AGATGTGAATCCGCTTGTCC	TTCGGCAATTGTCGCTTCTA	50.0	45.0	58.0	57.9	1649 bp	0.5	59.1