Hotspot

rpl32-ycf1

IRb · 114589-115462 · 874 bp

Back to Halophila

Pi 0.281

MarkerSeek score 81.40

Primer available yes

Label rpl32-ycf1

Feature Metadata

label_name: rpl32-ycf1
feature_type: igs
region: IRb
length_bp: 874
variable_sites: 211
pi: 0.281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf1_p1	ATGGGTTTACTCCACCAGGA	TGTGGCAAACGCAATTTTGT	50.0	40.0	57.9	58.0	1938-2914 bp	1.0	91.4
rpl32-ycf1_p2	ATGGGTTTACTCCACCAGGA	ATTCCCTAGGACCCAACCAA	50.0	50.0	57.9	57.9	1968-2944 bp	1.0	91.0
rpl32-ycf1_p3	AACATTTGTTGCCTGATGCG	TGTGGCAAACGCAATTTTGT	45.0	40.0	57.9	58.0	1659 bp	0.5	59.3
rpl32-ycf1_p4	ATGGGTTTACTCCACCAGGA	ACCGCGAATTTATGAGGTCC	50.0	50.0	57.9	58.1	1646 bp	0.5	59.2
rpl32-ycf1_p5	AACATTTGTTGCCTGATGCG	ACCGCGAATTTATGAGGTCC	45.0	50.0	57.9	58.1	1367 bp	0.5	59.0