Hotspot

ycf2-rps7

IRb · 100606-101331 · 726 bp

Back to Halophila

Pi 0.17

MarkerSeek score 80.40

Primer available yes

Label ycf2-rps7

Feature Metadata

label_name: ycf2-rps7
feature_type: igs
region: IRb
length_bp: 726
variable_sites: 102
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-rps7_p1	TCAGCAACCCAAAGTCGAAA	CGAGCCGTGAAAGAGATTCA	45.0	50.0	58.0	58.0	1205 bp	0.5	59.6
ycf2-rps7_p2	TTCAGCAACCCAAAGTCGAA	CGAGCCGTGAAAGAGATTCA	45.0	50.0	58.0	58.0	1206 bp	0.5	59.6
ycf2-rps7_p3	TCAGCAACCCAAAGTCGAAA	CTGTGGAAAGCCGTATTCGA	45.0	50.0	58.0	58.0	1500 bp	0.5	59.6
ycf2-rps7_p4	TCAGCAACCCAAAGTCGAAA	TCTGTGGAAAGCCGTATTCG	45.0	50.0	58.0	58.0	1501 bp	0.5	59.6
ycf2-rps7_p5	TTCAGCAACCCAAAGTCGAA	CTGTGGAAAGCCGTATTCGA	45.0	50.0	58.0	58.0	1501 bp	0.5	59.6