Hotspot

trnH-GUG-rpoA

IRb · 81010-81067 · 58 bp

Back to Halophila

Pi 0.0877
MarkerSeek score 76.60
Primer available yes
Label trnH-GUG-rpoA

Feature Metadata

label_name
trnH-GUG-rpoA
feature_type
igs
region
IRb
length_bp
58
variable_sites
5
pi
0.0877
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpoA_p1 TGGATTAAGGCGGTGGATTG CGTTTCGCAATTGATTTACCT 50.0 38.1 57.9 55.5 158-159 bp 1.0 54.7
trnH-GUG-rpoA_p2 GGATTAAGGCGGTGGATTGT CGTTTCGCAATTGATTTACCT 50.0 38.1 57.9 55.5 157-158 bp 1.0 54.7
trnH-GUG-rpoA_p3 GTGGATTAAGGCGGTGGATT CGTTTCGCAATTGATTTACCT 50.0 38.1 57.9 55.5 159-160 bp 1.0 54.7
trnH-GUG-rpoA_p4 GATACATTCCAAGGGACGGG CGTTTCGCAATTGATTTACCT 55.0 38.1 57.7 55.5 192-193 bp 1.0 52.6
trnH-GUG-rpoA_p5 CGGATGTAGCCAAGTGGATT CGTTTCGCAATTGATTTACCT 50.0 38.1 57.7 55.5 172-173 bp 1.0 52.2