Hotspot

trnD-GUC-trnY-GUA

LSC · 33479-33936 · 458 bp

Back to Halophila

Pi 0.0515

MarkerSeek score 76.40

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 21
pi: 0.0515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	488-528 bp	1.0	81.6
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	487-527 bp	1.0	81.6
trnD-GUC-trnY-GUA_p3	CGAATCCTAGTTCGGGACTG	TGGCAATATGTCTACGCTGG	55.0	50.0	57.5	57.8	544-584 bp	1.0	81.1
trnD-GUC-trnY-GUA_p4	CGAATCCTAGTTCGGGACTG	GGCAATATGTCTACGCTGGT	55.0	50.0	57.5	57.8	543-583 bp	1.0	81.1
trnD-GUC-trnY-GUA_p5	ATCCTAGTTCGGGACTGACG	TGGCAATATGTCTACGCTGG	55.0	50.0	58.6	57.8	541-581 bp	1.0	80.2