Hotspot

atpH-atpI

LSC · 15309-16245 · 937 bp

Back to Halophila

Pi 0.0392

MarkerSeek score 81.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 36
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGTCCAACTCCAGGT	TAGGTGAATCCTTGGAGGGT	50.0	50.0	57.9	57.3	1054-1055 bp	1.0	77.9
atpH-atpI_p2	GCAAGTCCTACGGCTAATCC	TAGGTGAATCCTTGGAGGGT	55.0	50.0	58.1	57.3	1025-1026 bp	1.0	77.9
atpH-atpI_p3	AGAAGCAAGTCCTACGGCTA	TAGGTGAATCCTTGGAGGGT	50.0	50.0	58.2	57.3	1029-1030 bp	1.0	77.7
atpH-atpI_p4	TAGAAGCAAGTCCTACGGCT	TAGGTGAATCCTTGGAGGGT	50.0	50.0	58.2	57.3	1030-1031 bp	1.0	77.7
atpH-atpI_p5	TACCTTGTCCAACTCCAGGT	AGGTGAATCCTTGGAGGGTC	50.0	55.0	57.9	58.7	1053-1054 bp	1.0	77.5