Hotspot

rrn4.5-rrn23

IRa · 129749-129849 · 101 bp

Back to Halogeton

Pi 0.0808

MarkerSeek score 83.40

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 101
variable_sites: 8
pi: 0.0808
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	194-198 bp	1.0	82.9
rrn4.5-rrn23_p2	TCACTGCACTTCCACTTGAC	TAGTAAGCCCACCCCAAGAT	50.0	50.0	58.0	57.7	191-195 bp	1.0	82.8
rrn4.5-rrn23_p3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	220-224 bp	1.0	82.3
rrn4.5-rrn23_p4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	222-226 bp	1.0	82.2
rrn4.5-rrn23_p5	CTGTTAGGATGCCTCAGCTG	TAGTAAGCCCACCCCAAGAT	55.0	50.0	58.0	57.7	217-221 bp	1.0	82.1