Hotspot

trnN-GUU-ndhF

IRb · 107197-108648 · 1452 bp

Back to Halogeton

Pi 0.0187

MarkerSeek score 65.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1452
variable_sites: 26
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GTATGGAGGAGGGAGTCGAA	50.0	55.0	58.2	57.9	1565-1605 bp	1.0	82.5
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	AAGTATGGAGGAGGGAGTCG	50.0	55.0	58.2	57.9	1567-1607 bp	1.0	82.4
trnN-GUU-ndhF_p3	AATCAGTTAACAGCCGACCG	GTATGGAGGAGGGAGTCGAA	50.0	55.0	58.3	57.9	1546-1586 bp	1.0	81.6
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	AAGTATGGAGGAGGGAGTCG	50.0	55.0	58.3	57.9	1548-1588 bp	1.0	81.5
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	GTATGGAGGAGGGAGTCGAA	55.0	55.0	57.7	57.9	1573-1613 bp	1.0	81.1