Hotspot

rpl36-infA

LSC · 79355-79476 · 122 bp

Back to Halogeton

Pi 0.0439

MarkerSeek score 72.30

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 5
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	AATTGGACGAACAGAAGCCC	TTATCGACTCCGCAACAAGG	50.0	50.0	58.5	58.0	178-186 bp	1.0	82.0
rpl36-infA_p2	AATTGGACGAACAGAAGCCC	GACTCCGCAACAAGGATTCA	50.0	50.0	58.5	58.2	173-181 bp	1.0	80.6
rpl36-infA_p3	TGGACGAACAGAAGCCCTTA	TTATCGACTCCGCAACAAGG	50.0	50.0	58.7	58.0	175-183 bp	1.0	80.5
rpl36-infA_p4	GGACGAACAGAAGCCCTTAT	TTATCGACTCCGCAACAAGG	50.0	50.0	57.3	58.0	174-182 bp	1.0	80.3
rpl36-infA_p5	AATTGGACGAACAGAAGCCC	ACTCCGCAACAAGGATTCAA	50.0	45.0	58.5	57.7	172-180 bp	1.0	80.0