Hotspot

psbE-petL

LSC · 64276-65510 · 1235 bp

Back to Halogeton

Pi 0.0309

MarkerSeek score 62.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 38
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAGTGGTCGTGCTAGAGAC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	2470-2488 bp	1.0	81.6
psbE-petL_p2	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2021-2034 bp	1.0	81.6
psbE-petL_p3	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2263-2276 bp	1.0	81.5
psbE-petL_p4	TTCGGGTTTGATTGTGTCGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2260-2273 bp	1.0	81.5
psbE-petL_p5	CAAGTGGTCGTGCTAGAGAC	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	2448-2466 bp	1.0	81.4