Hotspot

trnD-GUC-trnY-GUA

LSC · 29773-30144 · 372 bp

Back to Halogeton

Pi 0.036
MarkerSeek score 70.60
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
372
variable_sites
13
pi
0.036
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 443-507 bp 1.0 77.7
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 442-506 bp 1.0 77.7
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 464-528 bp 1.0 76.7
trnD-GUC-trnY-GUA_p4 GCGGTGCTCTAACCAATTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.6 57.6 463-527 bp 1.0 76.5
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTAACCAATTGA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.6 58.4 497-561 bp 1.0 75.9