Hotspot

petN-psbM

LSC · 28156-28876 · 721 bp

Back to Halogeton

Pi 0.0329

MarkerSeek score 70.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 23
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TGTAAAAACGGTCAGCCAAA	50.0	40.0	57.6	56.1	759-771 bp	1.0	66.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	ATGTAAAAACGGTCAGCCAA	50.0	40.0	57.6	55.9	760-772 bp	1.0	64.7
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGTAAAAACGGTCAGCCAAAA	50.0	38.1	57.6	56.9	759-771 bp	1.0	64.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TCTATGTAAAAACGGTCAGCCA	50.0	40.9	57.6	57.7	763-775 bp	1.0	63.6
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	ATGTAAAAACGGTCAGCCAAA	50.0	38.1	57.6	56.6	760-772 bp	1.0	62.9