Hotspot

psbI-trnS-GCU

LSC · 7617-7813 · 197 bp

Back to Halogeton

Pi 0.0632

MarkerSeek score 73.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 12
pi: 0.0632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGGCGCGAAGAATA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	59.5	57.0	238-240 bp	1.0	66.4
psbI-trnS-GCU_p2	ATCCTGGGCGCGAAGAATAA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	59.5	57.0	237-239 bp	1.0	66.4
psbI-trnS-GCU_p3	TAATCCTGGGCGCGAAGAAT	TTCGAATCCCTCTCTTTCCG	50.0	50.0	59.5	57.0	239-241 bp	1.0	66.4
psbI-trnS-GCU_p4	AATCCTGGGCGCGAAGAATA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	59.5	56.9	241-243 bp	1.0	65.4
psbI-trnS-GCU_p5	ATCCTGGGCGCGAAGAATAA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	59.5	56.9	240-242 bp	1.0	65.4