Hotspot

psbA-trnK-UUU

LSC · 1358-1581 · 224 bp

Back to Halogeton

Pi 0.0457

MarkerSeek score 78.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 10
pi: 0.0457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ATAGGCTTTCACTTTCGCGT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.9	57.9	379-383 bp	1.0	81.3
psbA-trnK-UUU_p2	ATAGGCTTTCACTTTCGCGT	CGGTTTGGGGAGAGGTTTTT	45.0	50.0	57.9	58.3	349-353 bp	1.0	80.3
psbA-trnK-UUU_p3	ATAGGCTTTCACTTTCGCGT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.9	58.4	361-365 bp	1.0	79.2
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.7	57.9	420-424 bp	1.0	79.1
psbA-trnK-UUU_p5	CATAGGCTTTCACTTTCGCG	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.6	57.9	380-384 bp	1.0	78.5