Hotspot

ycf1

SSC · 123021-128471 · 5451 bp

Back to Halimocnemis

Pi 0.0387

MarkerSeek score 61.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 210
pi: 0.0387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	37.5	45.0	56.0	57.2	167 bp	1.0	47.7
ycf1_p2	AGGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	36.0	45.0	57.4	57.2	168 bp	1.0	47.6
ycf1_p3	AGGATCTTTTATGATCAATCCCTC	ATGAAACTGGAACCAACCCA	37.5	45.0	56.0	57.2	168 bp	1.0	47.6
ycf1_p4	AAGGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	34.6	45.0	57.9	57.2	169 bp	1.0	47.6
ycf1_p5	AGGATCTTTTATGATCAATCCCTCTT	ATGAAACTGGAACCAACCCA	34.6	45.0	57.9	57.2	168 bp	1.0	47.6