Hotspot

rpl32-trnL-UAG

SSC · 112569-113355 · 787 bp

Back to Halimocnemis

Pi 0.0674
MarkerSeek score 84.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
787
variable_sites
51
pi
0.0674
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCCTTTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 57.9 58.1 912-913 bp 1.0 85.3
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCCTTTT CTCTCGCACTGCTTCCTAAG 45.0 55.0 57.9 58.1 914-915 bp 1.0 85.3
rpl32-trnL-UAG_p3 GGCAGCGTTAAAAGCCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 901-902 bp 1.0 85.0
rpl32-trnL-UAG_p4 GGCAGCGTTAAAAGCCTTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.9 57.8 894-895 bp 1.0 84.6
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.9 57.8 899-900 bp 1.0 84.4