Hotspot

trnN-GUU-ndhF

IRb · 106960-109071 · 2112 bp

Back to Halimocnemis

Pi 0.0401

MarkerSeek score 73.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2112
variable_sites: 77
pi: 0.0401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GGCAGAATAGCAGAGCAAGT	ATTCCGAATGGCTTTGGTGT	50.0	45.0	58.0	58.1	2343-2503 bp	1.0	85.9
trnN-GUU-ndhF_p2	TGGGTCTTTTGTCGAAGTGG	ATTCCGAATGGCTTTGGTGT	50.0	45.0	58.0	58.1	2453-2615 bp	1.0	85.8
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	TGGCTTTGGTGTTACAAGCT	50.0	45.0	58.0	57.9	2495 bp	0.5	59.2
trnN-GUU-ndhF_p4	TGGGTCTTTTGTCGAAGTGG	TGGCTTTGGTGTTACAAGCT	50.0	45.0	58.0	57.9	2607 bp	0.5	59.2
trnN-GUU-ndhF_p5	GCTTCCCTACTCTTAACGGC	TGGCTTTGGTGTTACAAGCT	55.0	45.0	58.1	57.9	2390 bp	0.5	59.0