Hotspot

trnP-UGG-psaJ

LSC · 66447-66903 · 457 bp

Back to Halimocnemis

Pi 0.0435

MarkerSeek score 82.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 19
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	544-547 bp	1.0	84.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	543-546 bp	1.0	84.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	555-558 bp	1.0	84.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	554-557 bp	1.0	84.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	557-560 bp	1.0	84.4