Hotspot

accD-psaI

LSC · 58685-59273 · 589 bp

Back to Halimocnemis

Pi 0.052

MarkerSeek score 75.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 30
pi: 0.052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	854-863 bp	1.0	84.0
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1024-1033 bp	1.0	83.4
accD-psaI_p3	AACGCCTACATTGCATTTGC	CATGGGGGCTCGTTCATTTA	45.0	50.0	58.0	57.9	1018-1027 bp	1.0	83.4
accD-psaI_p4	AACGCCTACATTGCATTTGC	TGGGGGCTCGTTCATTTATG	45.0	50.0	58.0	57.9	1016-1025 bp	1.0	83.4
accD-psaI_p5	AACGCCTACATTGCATTTGC	GGGGGCTCGTTCATTTATGT	45.0	50.0	58.0	57.9	1015-1024 bp	1.0	83.4