Hotspot

trnG-UCC-trnfM-CAU

LSC · 36341-36570 · 230 bp

Back to Halimocnemis

Pi 0.0711
MarkerSeek score 77.60
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
230
variable_sites
16
pi
0.0711
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG GTTCAAATCCTGTCTCCGCA 50.0 50.0 57.8 58.2 295-296 bp 1.0 82.8
trnG-UCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 328-329 bp 1.0 82.0
trnG-UCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 329-330 bp 1.0 81.9
trnG-UCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG TCAAATCCTGTCTCCGCAAA 50.0 45.0 57.8 57.4 293-294 bp 1.0 79.9
trnG-UCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG TTCAAATCCTGTCTCCGCAA 50.0 45.0 57.8 57.4 294-295 bp 1.0 79.9