Hotspot

petN-psbM

LSC · 28173-28871 · 699 bp

Back to Halimocnemis

Pi 0.0418

MarkerSeek score 75.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 29
pi: 0.0418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	50.0	40.0	58.3	56.4	807-821 bp	1.0	68.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	50.0	40.0	57.6	56.4	745-759 bp	1.0	68.4
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	50.0	40.0	58.3	56.2	806-820 bp	1.0	67.0
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	50.0	40.0	58.3	56.2	805-819 bp	1.0	67.0
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	50.0	38.1	58.3	57.2	808-822 bp	1.0	66.8