Hotspot

rps3

LSC · 86888-87544 · 657 bp

Back to Halesia

Pi 0.00761

MarkerSeek score 63.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 5
pi: 0.00761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GGGGTAGAAAGAAAAGACAAGGA	TCGAGCTCGAGGACGTAATT	43.5	50.0	58.0	58.6	804-805 bp	1.0	51.5
rps3_p2	ACTGGGGTAGAAAGAAAAGACA	TCGAGCTCGAGGACGTAATT	40.9	50.0	56.9	58.6	807-808 bp	1.0	51.1
rps3_p3	TGGGGTAGAAAGAAAAGACAAGG	TCGAGCTCGAGGACGTAATT	43.5	50.0	58.3	58.6	805-806 bp	1.0	49.4
rps3_p4	ACTGGGGTAGAAAGAAAAGACAA	TCGAGCTCGAGGACGTAATT	39.1	50.0	57.6	58.6	807-808 bp	1.0	48.5
rps3_p5	GGGGTAGAAAGAAAAGACAAGG	TCGAGCTCGAGGACGTAATT	45.5	50.0	56.6	58.6	804-805 bp	1.0	48.5