Hotspot

ndhA

SSC · 124214-126412 · 2199 bp

Back to Halesia

Pi 0.00776

MarkerSeek score 57.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2199
variable_sites: 17
pi: 0.00776
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTGATGTTTGAGACAAATCGTG	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.9	56.5	2261-2270 bp	1.0	44.1
ndhA_p2	GTGATGTTTGAGACAAATCGTGA	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.7	56.5	2260-2269 bp	1.0	42.1
ndhA_p3	TGTGATGTTTGAGACAAATCGTG	TGGGAGAAGTTGATCGTTGAA	39.1	42.9	57.9	57.2	2261-2270 bp	1.0	42.1
ndhA_p4	TGTGATGTTTGAGACAAATCGT	TGGGAGAAGTTGATCGTTGA	36.4	45.0	56.6	56.5	2261-2270 bp	1.0	41.4
ndhA_p5	TGTGATGTTTGAGACAAATCGTG	ATGGGAGAAGTTGATCGTTGA	39.1	42.9	57.9	57.0	2262-2271 bp	1.0	40.9