Hotspot

rps15-ycf1

SSC · 127961-128339 · 379 bp

Back to Halesia

Pi 0.0185

MarkerSeek score 64.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 379
variable_sites: 7
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCAACAGATCCCCGA	CCCGCGATTGAAAATTCGTT	42.9	45.0	57.3	57.7	475 bp	1.0	65.4
rps15-ycf1_p2	TCAACAGATCCCCGATTTTCT	CCCGCGATTGAAAATTCGTT	42.9	45.0	57.0	57.7	469 bp	1.0	63.1
rps15-ycf1_p3	TTGAAATTCAACAGATCCCCGA	CCCGCGATTGAAAATTCGTT	40.9	45.0	58.0	57.7	476 bp	1.0	62.9
rps15-ycf1_p4	TGAAATTCAACAGATCCCCGAT	CCCGCGATTGAAAATTCGTT	40.9	45.0	57.8	57.7	475 bp	1.0	61.2
rps15-ycf1_p5	TGAAATTCAACAGATCCCCG	CCCGCGATTGAAAATTCGTT	45.0	45.0	55.7	57.7	475 bp	1.0	61.0