Hotspot

ndhF-rpl32

SSC · 116623-117578 · 956 bp

Back to Halesia

Pi 0.011

MarkerSeek score 57.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 956
variable_sites: 10
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACTCTGAAAAGCCCACAG	AAAACGCTTTTAAGGCTGCC	50.0	45.0	58.0	57.9	1138-1187 bp	1.0	82.9
ndhF-rpl32_p2	ACACTCTGAAAAGCCCACAG	GAAAATCCGAATTCGCACCC	50.0	50.0	58.0	57.8	1491-1540 bp	1.0	82.0
ndhF-rpl32_p3	ACACTCTGAAAAGCCCACAG	GGAAAATCCGAATTCGCACC	50.0	50.0	58.0	57.8	1492-1541 bp	1.0	82.0
ndhF-rpl32_p4	ACACTCTGAAAAGCCCACAG	GAAAGCGGGAGATATGTGGG	50.0	55.0	58.0	58.1	1735 bp	0.5	58.9
ndhF-rpl32_p5	CCCACAGACGACGAAGATTT	GAAAGCGGGAGATATGTGGG	50.0	55.0	57.9	58.1	1722 bp	0.5	58.4