Hotspot

rps12-clpP

LSC · 73852-73990 · 139 bp

Back to Halesia

Pi 0.0144

MarkerSeek score 60.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 2
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCGGGAGATTTCGTGACATT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.6	57.9	265 bp	1.0	74.6
rps12-clpP_p2	GAAGAGCGGGAGATTTCGTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.7	57.9	270 bp	1.0	73.9
rps12-clpP_p3	GTACATGTTCCTCGACGCTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.7	57.9	301 bp	1.0	73.8
rps12-clpP_p4	AGTACATGTTCCTCGACGCT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.8	57.9	302 bp	1.0	73.0
rps12-clpP_p5	TACATGTTCCTCGACGCTGA	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.8	57.9	300 bp	1.0	73.0