Hotspot

psbE-petL

LSC · 68554-69839 · 1286 bp

Back to Halesia

Pi 0.0101

MarkerSeek score 53.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 13
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.9	55.5	1446 bp	1.0	52.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.8	55.5	1369 bp	1.0	51.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GAGGTTATAGTTAAAGCCGCT	45.0	42.9	58.2	55.5	1455 bp	1.0	51.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	58.2	55.5	1460 bp	1.0	51.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.8	55.5	1454 bp	1.0	51.1