Hotspot

petA-psbJ

LSC · 66760-67780 · 1021 bp

Back to Halesia

Pi 0.00687

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 7
pi: 0.00687
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1163-1164 bp	1.0	76.3
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1164-1165 bp	1.0	76.2
petA-psbJ_p3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1158-1159 bp	1.0	73.5
petA-psbJ_p4	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1157-1158 bp	1.0	73.1
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCCGGATTGGG	50.0	47.6	58.2	57.8	1171-1172 bp	1.0	69.4