Hotspot

rbcL-accD

LSC · 59835-60517 · 683 bp

Back to Halesia

Pi 0.00746

MarkerSeek score 64.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 5
pi: 0.00746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CAACATCGAATTGAACCGCC	55.0	50.0	58.0	58.1	841-854 bp	1.0	80.5
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	AACATCGAATTGAACCGCCT	55.0	45.0	58.0	58.2	840-853 bp	1.0	80.0
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCGCCTT	55.0	45.0	58.0	58.2	839-852 bp	1.0	80.0
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CAACATCGAATTGAACCGCC	50.0	50.0	57.9	58.1	811-824 bp	1.0	80.0
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ACATCGAATTGAACCGCCTT	50.0	45.0	57.9	58.2	809-822 bp	1.0	79.4