Hotspot

trnT-GGU-psbD

LSC · 33643-35129 · 1487 bp

Back to Halesia

Pi 0.00605

MarkerSeek score 53.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1487
variable_sites: 9
pi: 0.00605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1637-1645 bp	1.0	78.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1640-1648 bp	1.0	77.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1636-1644 bp	1.0	76.8
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AGAGCAATAAACCCGACCAG	55.0	50.0	58.1	57.6	1660-1668 bp	1.0	76.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1639-1647 bp	1.0	76.6