Hotspot

rpoB-trnC-GCA

LSC · 27944-29054 · 1111 bp

Back to Halesia

Pi 0.00631

MarkerSeek score 62.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 7
pi: 0.00631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCTTCATTTCCATCCCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1177-1179 bp	1.0	74.5
rpoB-trnC-GCA_p2	TTCCTTCATTTCCATCCCGG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1178-1180 bp	1.0	74.3
rpoB-trnC-GCA_p3	GCCAAGCCCTGATCAATGAA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1248-1250 bp	1.0	73.6
rpoB-trnC-GCA_p4	TCCTTCATTTCCATCCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1202-1204 bp	1.0	73.5
rpoB-trnC-GCA_p5	TTCCTTCATTTCCATCCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1203-1205 bp	1.0	73.3