Hotspot

rps16-trnQ-UUG

LSC · 6321-8054 · 1734 bp

Back to Halesia

Pi 0.00635
MarkerSeek score 56.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1734
variable_sites
11
pi
0.00635
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1814-1824 bp 1.0 79.9
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1816-1826 bp 1.0 78.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1807-1817 bp 1.0 78.9
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1817-1827 bp 1.0 78.2
rps16-trnQ-UUG_p5 GTCTTTCAAGTCGCACGTTG AAATTTAAATGGGGCGTGGC 50.0 45.0 58.0 57.6 1871-1881 bp 1.0 77.4