Hotspot

rpl32-trnL-UAG

SSC · 116109-116868 · 760 bp

Back to Halerpestes

Pi 0.18

MarkerSeek score 82.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 121
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATGACGAGAATACGCGGAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1245 bp	0.5	58.0
rpl32-trnL-UAG_p2	CATGACGAGAATACGCGGAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1246 bp	0.5	57.7
rpl32-trnL-UAG_p3	AACCTTTCCTCGAACTTCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	1079 bp	0.5	57.7
rpl32-trnL-UAG_p4	ATGACGAGAATACGCGGAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	1238 bp	0.5	57.6
rpl32-trnL-UAG_p5	ATGACGAGAATACGCGGAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	1243 bp	0.5	57.5