Hotspot

ndhF-rpl32

SSC · 115163-115946 · 784 bp

Back to Halerpestes

Pi 0.205

MarkerSeek score 83.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 149
pi: 0.205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCCGAAATTCAAAACTGCCC	TTTGCTGCCCAGTATGCTTT	50.0	45.0	57.9	58.4	1489 bp	0.5	56.4
ndhF-rpl32_p2	CCCGAAATTCAAAACTGCCC	TTTTGCTGCCCAGTATGCTT	50.0	45.0	57.9	58.4	1490 bp	0.5	56.4
ndhF-rpl32_p3	CCCGAAATTCAAAACTGCCC	TTTTTGCTGCCCAGTATGCT	50.0	45.0	57.9	58.4	1491 bp	0.5	56.4
ndhF-rpl32_p4	GACGGGAAAAGGAGAAGACC	TTTGCTGCCCAGTATGCTTT	55.0	45.0	57.9	58.4	962 bp	0.5	56.3
ndhF-rpl32_p5	GACGGGAAAAGGAGAAGACC	TTTTGCTGCCCAGTATGCTT	55.0	45.0	57.9	58.4	963 bp	0.5	56.3