Hotspot

rbcL-accD

LSC · 58121-58866 · 746 bp

Back to Halerpestes

Pi 0.106

MarkerSeek score 84.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 74
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	CCGAATGTTGCTGACACCTA	50.0	50.0	58.0	57.9	1058-1085 bp	1.0	89.5
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	TCTTCACCCCCATCGGTATT	50.0	50.0	58.0	58.1	1067-1094 bp	1.0	89.4
rbcL-accD_p3	ACTACAGTTCGGTGGAGGAA	CCGAATGTTGCTGACACCTA	50.0	50.0	58.0	57.9	1155-1182 bp	1.0	89.0
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	CCGAATGTTGCTGACACCTA	50.0	50.0	58.0	57.9	1634-1661 bp	1.0	86.7
rbcL-accD_p5	AGAGAGATCACCTTGGGCTT	CCGAATGTTGCTGACACCTA	50.0	50.0	58.0	57.9	1364 bp	0.5	59.0