Hotspot

ndhC-trnV-UAC

LSC · 52550-53015 · 466 bp

Back to Halerpestes

Pi 0.309

MarkerSeek score 83.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 140
pi: 0.309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	522-1412 bp	1.0	56.4
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	521-1411 bp	1.0	53.7
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	510-1400 bp	1.0	52.5
ndhC-trnV-UAC_p4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	521-1411 bp	1.0	52.4
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.6	57.8	522-1412 bp	1.0	52.4